Stem cell therapy is an emerging treatment modality for various diseases.\nBecause mesenchymal stem cells (MSCs) are known to accumulate at the site of damage, their possible\nclinical application has been investigated. MSCs are usually administered using intravenous\ninjection, but this route carries a risk of pulmonary embolism. In contrast, topical injection of\nMSCs reportedly has an inferior therapeutic effect. We developed a remote administration method\nthat uses collagen gel as a scaffold and investigated the effect of this scaffold on the retention of\nstemness, homing ability, and therapeutic effect using a mouse tooth extraction model. After verifying\nthe retention of stemness of MSCs isolated from the bone marrow of donor mice in the scaffold,\nwe administered MSCs subcutaneously into the back of the recipient mice with scaffold and observed\nthe accumulation and the acceleration of healing of the extraction socket of the maxillary first molar.\nThe MSCs cultured with scaffold retained stemness, the MSCs injected into back skin with scaffold\nsuccessfully accumulated around the extraction socket, and socket healing was significantly enhanced.\nIn conclusion, administration of MSCs with collagen scaffold at a remote site enhanced the lesion\nhealing without the drawbacks of currently used administration methods.
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